Analysis of Foot and Mouth Disease Virus Polyprotein for Multi Peptides Vaccine Design: An In silico Strategy

Foot-and-mouth disease virus (FMDV) is small RNA from Picornaviridae family; genus Aphthovirus. FMDV causes maximum levels of infectivity in cattle and harmful socioeconomic effects. The present report attempted to design vaccine candidate the polyprotein stimulate protective immune response. IEDB server was used predict B T cells epitopes that were linked via GPGPG YAA linkers, respectively. Mycobacterium tuberculosis 50S ribosomal protein exploited as an adjuvant a six histidine-tag sequence carboxyl end for purification identification. predicted comprised 313aa antigenic not allergic. Moreover, acidic showed stability hydrophilicity. Vaccine secondary tertiary structures predicted. structure refined ameliorate quality global local vaccine. model validation performed final score structural computed. validated molecular docking with bovine (N*01801-BoLA-A11) allele. Docking process terms binding free energy significant. solubility investigated based on E. coli disulfide bonding lessen entropic mobile points Lastly, silico cloning ensured proper best translation DNA vectors.